I’m very excited to share the first big project from my PhD (my first proper-first, yay!) where we use cryoET and a fun new flavour of CLEM to study the re-organisation of photosynthetic machinery of cyanobacteria growing beyond the red limit of light. doi.org/10.64898/202...

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www.biorxiv.org/content/10.6...

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Interviewer: Can you explain this gap in your CV? Scientist: I was trying to move a figure in Microsoft Word

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Why a phase plate? Biological samples are weak-phase objects, so in-focus images show almost no contrast. Defocus helps, but at the cost of high-resolution information. The xLPP shifts the phase of the unscattered beam by 90° instead. Left: no LPP, 2–3 µm defocus. Right: xLPP, 0.5–1.5 µm 🔴

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New on the #cryoET Data Portal: three datasets benchmarking the crossed laser phase plate (xLPP) ⚡ Data by Reza Paraan and the @biohub.org team with collaborators at UC Berkeley. Full credits at the end of the 🧵 cryoetdataportal.czscience.com/depositions/10363

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