📢 Publication Alert 📢 : Congratulations to Ana from the @jesperolsenlab.bsky.social for proteomic analysis of this cohort of colorectal cancers! Providing insights into CAVIN1 in tumour relapse! Thank you for trusting us with your sample preparation www.embopress.org/doi/full/10....

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We are using limma based R scripts to analyse our proteomics data. One annoying thing that we often saw is that multiple proteins appeared to have the same adjusted p-value. This leads to "streaks" of proteins in volcano plots. 1/2

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As the technical advances significantly improve the outputs from SCP to interrogate around 5k proteins per cell, the next step is addressing the logistics to improve its adoption.. Formaldehyde-fixation to maintain cell integrity prior to SCP.. A nice study comparing fresh and fixed cells..

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Identification numbers and FDR control of Spectronaut 18 vs DIA-NN 1.9 as benchmarked by Jesper Olsen and colleagues (Nature Methods) www.nature.com/articles/s41...

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Fantastic update on advances in single cell proteomics from @jesperolsenlab.bsky.social

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