Luciano A. Masullo
@lumasullo.bsky.social did:plc:zgfpkd2quew4okvnyldpoxt4
Physicist | single molecules, biotech, nanotech, optics, fluorescence, glycans, immunology, cell signalling | Group Leader at Glycoscience Program IBYME
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Luciano A. Masullo (@lumasullo.bsky.social) é um usuário do Bluesky desde 10 novembro 2024. Tem 850 seguidores, segue 386 contas e publicou 77 posts.
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Very excited to share our newest paper on finding positions of molecules in DNA-PAINT! G5M extracts more information from the data than previous tools, allowing for accurate molecular mapping.
Ver postWe hope G5M pushes molecular mapping forward. Excited to see the discoveries it enables! Thanks to @rafalkowalew.bsky.social, @susannereinhardt.bsky.social, @ipachmayr.bsky.social, Shuhan Xu, @lumasullo.bsky.social for developing G5M and the @jungmannlab.bsky.social for testing and improving it. 7/7
Ver postG5M is implemented in Picasso since version 0.9.5: github.com/jungmannlab/..., although we always recommend using the most recent version! A short documentation: picassosr.readthedocs.io/en/latest/re.... We advise reading the whole paper for best results! 6/7
Ver postBonus: we found a new formula for axial loc. precision for astigmatic imaging for any z position. It only depends on the 3D calibration and the properties of localizations. Interestingly, the results are not necessarily symmetric around z = 0. 5/7
Ver postWe validated G5M on DNA origami, Nuclear Pore Complexes (NPCs) and Obinutuzumab-treated CD20 RESI data. In NPCs, G5M found twice as many Nup96 dimers as GA, demonstrating increased accuracy in molecular mapping. 4/7
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