Luciano A. Masullo
lumasullo.bsky.social
Physicist | single molecules, biotech, nanotech, optics, fluorescence, glycans, immunology, cell signalling | Group Leader at Glycoscience Program IBYME
👤 Joined on 10 November 2024
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- 10 Kas 2024, 11:49
🎉 Bluesky'e katıldı - İlk kullanıcı adı:
@lumasullo.bsky.social
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Very excited to share our newest paper on finding positions of molecules in DNA-PAINT! G5M extracts more information from the data than previous tools, allowing for accurate molecular mapping.
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We hope G5M pushes molecular mapping forward. Excited to see the discoveries it enables! Thanks to @rafalkowalew.bsky.social, @susannereinhardt.bsky.social, @ipachmayr.bsky.social, Shuhan Xu, @lumasullo.bsky.social for developing G5M and the @jungmannlab.bsky.social for testing and improving it. 7/7
Show PostG5M is implemented in Picasso since version 0.9.5: github.com/jungmannlab/..., although we always recommend using the most recent version! A short documentation: picassosr.readthedocs.io/en/latest/re.... We advise reading the whole paper for best results! 6/7
Show PostBonus: we found a new formula for axial loc. precision for astigmatic imaging for any z position. It only depends on the 3D calibration and the properties of localizations. Interestingly, the results are not necessarily symmetric around z = 0. 5/7
Show PostWe validated G5M on DNA origami, Nuclear Pore Complexes (NPCs) and Obinutuzumab-treated CD20 RESI data. In NPCs, G5M found twice as many Nup96 dimers as GA, demonstrating increased accuracy in molecular mapping. 4/7
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