Nezar Abdennur

nvictus.bsky.social

21 September 2026 15:09

And a huge thanks to the Mirny, Maehr, and Dekker labs, as well as the feedback and support of our multi-PI center over the course of Phase II of the 4DN Consortium.

Nezar Abdennur

nvictus.bsky.social

21 September 2026 15:09

This all was no small feat, but the outcome of tireless effort across a team spanning 4 labs! Congratulations to all involved, including lead authors Xiangru, Denis, Thomas and Liyan, as well as Johan Gibcus, @renemaehr.bsky.social , @leonidmirny.bsky.social , and @jobdekker.bsky.social

Nezar Abdennur

nvictus.bsky.social

21 September 2026 15:08

These transitions are also tied to regulation. Using joint decomposition of long-range interactions across all five stages, we isolated a trajectory where H3K27me3 changes from sharp peaks in to broad domains covering coinciding with silencing of genes marking alternative, non-hepatic lineages.

Nezar Abdennur

nvictus.bsky.social

21 September 2026 15:08

3. LCHi-C shows that chromatin interactions stabilize over differentiation and do so in a defined order: speckle-associated domains harden at commitment, while the rest of the genome hardens during maturation.

Nezar Abdennur

nvictus.bsky.social

21 September 2026 15:08

2. At hepatic commitment, compartmentalization increases dramatically, driven by segregation of speckle-associated from lamina-associated chromatin. H3K9me2 gets deposited and LADs peripheralize, speckles consolidate, and B-type lamins get replaced by lamin A/C.