@ptrrupprecht.bsky.social can you give an estimate for the difference in baseline brightness between GCaMP8s and 6f?

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the hivemind is of course also invited to answer (eg. @loogerl.bsky.social )

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Another thing we learned. Blue light resistance is easy to break. We found a few variants along the way that made blue light resistance worse. A clear takeaway is that this is something that should be screened for up front, rather than just optimizing for response sizes.

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We’re now testing ScaRCaMP-2.0 in vivo and are working hard to continue to optimize this to create a better tool for all-optical experiments. This is still a work in progress, but we hope this provides a new path for blue-light resistant biosensors! Reach out to us if you want to try this out!

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We simulated possible structures of our sensor and @dkoveal.bsky.social noticed a possible mechanism that looks distinct from other GECIs. We used this to further optimize our sensor to create ScaRCaMP-2.0, which has double the response size of ScaRCaMP-1.0 and retains its blue-light resistance.

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