What an exciting scientific journey!

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#BSI25 Really interesting seminar on NK cell clonality by @romagnanilab.bsky.social with work from @timorckert.bsky.social Mindblowing proportion of NK cells derived from a single clone!

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For our #flowcytometry peeps, would you like to have a single fix/perm protocol that is optimised for everything? One that preserves fluorophores while allowing simultaneous TF and cytokine staining? How about 100-fold cheaper? You got it: currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...

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Super-large targeted Knock-Ins in primary T cells, with multigene inserts >8kb, in a single reaction. HOW?? 🙀 Combine simple CRISPR-HDR & Integrases for one-pot PASTA🍝 = Programmable And Site-specific Transgene Addition. 😎

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You like engineering T cells, but you are missing the right flavor? Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1 We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt 𝐏rogrammable 𝐀nd 𝐒ite-specific 𝐓ransgene 𝐀ddition 🧪

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